期刊
GENETICS
卷 185, 期 2, 页码 417-U56出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.110.116319
关键词
-
资金
- National Institutes of Health [R01NS039996-05, R01NS050266-03, NS054540-01]
- European Molecular Biology Organization
- Human Frontier Science Program Organization
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [F31NS054540, R01NS039996, R01NS050266] Funding Source: NIH RePORTER
Whole-genome sequencing (WGS) of organisms displaying a specific mutant phenotype is a powerful approach to identify the genetic determinants of a plethora of biological processes. We have previously validated the feasibility of this approach by identifying a point-mutated locus responsible for a specific phenotype, observed in an ethyl methanesulfonate (EMS)-mutagenized Caenorhabditis elegans strain. Here we describe the genome-wide mutational profile of 17 EMS-mutagenized genomes as assessed with a bioinformatic pipeline, called MAQGene. Surprisingly, we find that while outcrossing mutagenized strains does reduce the total number of mutations, a striking mutational load is still observed even in outcrossed strains. Such genetic complexity has to be taken into account when establishing a causative relationship between genotype and phenotype. Even though unintentional, the 17 sequenced strains described here provide a resource of allelic variants in almost 1000 genes, including 62 premature stop codons, which represent candidate knockout alleles that will be of further use for the C. elegans community to study gene function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据