期刊
GENETICS
卷 186, 期 4, 页码 1493-1496出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.110.123471
关键词
-
资金
- Selman A. Waksman Chair in Molecular Genetics at Rutgers University
Maize Mucronate1 is a dominant floury mutant based on a misfolded 16-kDa gamma-zein protein. To prove its function, we applied RNA interference (RNAi) as a dominant suppressor of the mutant seed phenotype. A gamma-zein RNAi transgene was able to rescue the mutation and restore normal seed phenotype. RNA interference prevents gene expression. In most cases, this is used to study gene function by creating a new phenotype. Here, we use it for the opposite purpose. We use it to reverse the creation of a mutant phenotype by restoring the normal phenotype. In the case of the maize Mucronate1 (Mc1) phenotype, interaction of a misfolded protein with other proteins is believed to be the basis for the Mc1 phenotype. If no misfolded protein is present, we can reverse the mutant to the normal phenotype. One can envision using this approach to study complex traits and in gene therapy.
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