期刊
GENETICS
卷 180, 期 1, 页码 133-145出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.108.091371
关键词
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资金
- University of Iowa [MDA4221]
- American Heart Association [0615504Z]
The nuclear lamina represents a protein network required for nuclear structure and function. One family of lamina proteins is defined by an similar to 40-aa LAP2, Emerin, and MAN1 (LEM) domain (LEM-D) that binds the nonspecific DNA-binding protein, barrier-to-autointegration factor (BAF). Through interactions with BAF, LEM-D proteins serve as a bridge between chromosomes and the nuclear envelope. Mutations in genes encoding LEM-D proteins cause human laminoathies that are associated with tissue-restricted pathologies. Drosophila has five genes that encode propteins with LEM homology. Using yeast two-hybrid analyses, we demonstrate that four encode proteins that bine Drosophila (d) BAF. In addition of dBAF, dMAN1 associates with lamins, the LEM-D protein Bocksbeutel, and the receptor-regulated Smads, demonstrating parallel protein interactions with vertebrate homologs. P-element mobilization was used to generate null dMAN1 alleles. These mutants showed decreased viability, with surviving adults displaying male sterility, decreased female fertility, wing patterning and positioning defects, flightlessness, and locomotion difficulties that became more severe with age. Increased phospho-Smad staining in staining in dMAN1 mutant wing discs is consistent with a role in transforming growth factor (TGF)-beta/bone morphogenic protein (BMP) signaling. The tissue-specific, age-enhanced dMAN1 mutant phenotypes are reminiscent of human laminopathies, suggesting that studies in Drosophila will provide insights into lamina dysfunction associated with disease.
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