期刊
GENESIS
卷 52, 期 10, 页码 858-863出版社
WILEY-BLACKWELL
DOI: 10.1002/dvg.22806
关键词
HoxB cluster; Hoxb4a; zebrafish; fluorescent fusion protein
资金
- National Institutes of Health grant USPHS [P50HG004071]
- Gordon Ross Postdoctoral Fellowship
- NIH [5T32NS007251]
We describe a novel zebrafish line that fluorescently tags a previously unknown protein, CT74a, allowing us to follow its endogenous expression in real time and at subcellular resolution in live embryos. Our results showed that CT74a-Citrine fusion protein is expressed in the developing pharyngeal arches, hind-brain, and fin buds in a pattern highly reminiscent of transcription factors belonging to anterior Hox gene families, including expression in a subset of neuronal nuclei. Consistent with this, splinkerette-PCR revealed that CT74a-Citrine's genomic integration is within the HoxB region, and 30 RACE demonstrated that its downstream coding sequence has no recognizable homology. Thus, CT74a is a previously unknown protein located within the HoxB cluster adjacent to Hoxb4a and is expressed in a Hoxb4a-like pattern. (C) 2014 Wiley Periodicals, Inc.
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