期刊
GENESIS
卷 50, 期 10, 页码 766-774出版社
WILEY-BLACKWELL
DOI: 10.1002/dvg.22036
关键词
conditional knockout; gene targeting; Cre recombination; Foxc1; Foxc2
资金
- US National Institutes of Health (NIH) [HL074121, EY019484]
The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell-type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole-organism) homozygous-null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional-null Foxc1flox and Foxc2flox mutations that are induced via Cre-mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild-type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre-deleter mice that are homozygous for the recombined allele (i.e., Foxc1?/? or Foxc2?/? embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell-type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals. genesis 50:766774, 2012. (c) 2012 Wiley Periodicals, Inc.
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