期刊
GENESIS
卷 50, 期 2, 页码 124-131出版社
WILEY
DOI: 10.1002/dvg.20810
关键词
prestin; Cre recombinase; inner ear; outer hair cells
资金
- National Organization for Hearing Research Foundation
- Hartwell Individual Biomedical Research Award
- National Institutes of Health [DC006471, DC008800, CA21765]
- Office of Naval Research [N000140911014]
- American Lebanese Syrian Associated Charities of St. Jude Children's Research Hospital
- National Natural Science Foundation of China [NSFC 30540420522]
Outer hair cells (OHCs) in the cochlea are crucial for the remarkable hearing sensitivity and frequency tuning. To understand OHC physiology and pathology, it is imperative to use mouse genetic tools to manipulate gene expression specifically in OHCs. Here, we generated two prestin knockin mouse lines: (1) the prestin-CreERT2 line, with an internal ribosome entry site-CreERT2-FRT-Neo-FRT cassette inserted into the prestin locus after the stop codon, and (2) the prestin-CreERT2-NN line, with the FRT-Neo-FRT removed subsequently. We characterized the inducible Cre activity of both lines by crossing them with the reporter lines CAG-eGFP and Ai6. Cre activity was induced with tamoxifen at various postnatal ages and only detected in OHCs, resembling the endogenous prestin expression pattern. Moreover, prestin-CreERT2 +/- (heterozygotes) and +/+ (homozygotes) as well as prestin-CreERT2-NN +/- mice displayed normal hearing. These two prestin-CreERT2 mouse lines are therefore useful tools to analyze gene function in OHCs in vivo. genesis 50:124131, 2012. (C) 2011 Wiley Periodicals, Inc.
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