期刊
GENESIS
卷 48, 期 8, 页码 492-504出版社
WILEY
DOI: 10.1002/dvg.20650
关键词
cardiogenesis; integrins; development; mitogen; Xenopus
资金
- National Heart, Lung, and Blood Institute [HL-081844, HL-071054, HL070953, HL 089641]
- American Heart Association [0355776U, 0555476U]
- National Institute of Dental and Craniofacial Research [DE-018825]
- National Institutes of Health [T32HL69768]
- NICHD
Focal adhesion kinase (FAK) is a critical mediator of matrix- and growth factor-induced signaling during development. Myocyte-restricted FAK deletion in mid-gestation mice results in impaired ventricular septation and cardiac compaction. However, whether FAK regulates early cardiogenic steps remains unknown. To explore a role for FAK in multi-chambered heart formation, we utilized anti-sense morpholinos to deplete FAK in Xenopus laevis. Xenopus FAK morphants exhibited impaired cardiogenesis, pronounced pericardial edema, and lethality by tadpole stages. Spatial-temporal assessment of cardiac marker gene expression revealed that FAK was not necessary for midline migration, differentiation, fusion of cardiac precursors, or linear heart tube formation. However, myocyte proliferation was significantly reduced in FAK morphant heart tubes and these tubes failed to undergo proper looping morphogenesis. Collectively our data imply that FAK plays an essential role in chamber outgrowth and looping morphogenesis likely stimulated by fibroblast growth factors (and possibly other) cardiotrophic factors. genesis 48:492-504,2010. (C) 2010 Wiley-Liss, Inc.
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