期刊
GENESIS
卷 47, 期 4, 页码 281-287出版社
WILEY-BLACKWELL
DOI: 10.1002/dvg.20497
关键词
IL-7; BAC transgene; Cre recombinase
资金
- NIH
- Welch Fdn
- MRC
- MRC [G0601156] Funding Source: UKRI
- Medical Research Council [G0601156] Funding Source: researchfish
IL-7 is a cytokine that is required for T-cell development and homeostasis as well as for lymph node organogenesis. Despite the importance of IL-7 in the immune system and its potential therapeutic relevance, questions remain regarding the sites of IL-7 synthesis specific cell types involved and molecular mechanisms regulating IL-7 expression. To address these issues, we generated two bacterial artificial chromosome (BAC) transgenic mouse lines in which IL-7 regulatory elements drive expression of either Cre recombinase or a human CD25 (hCD25) cell surface reporter molecule. Expression of the IL-7.hCD25 BAC transgene, detected by reactivity with anti-hCD25 antibody, mimicked endogenous IL-7 expression. Fetal and adult tissues from crosses between IL-7.Cre transgenic mice and Rosa26R or R26-EYFP reporters demonstrated X-gal or YFP staining in tissues known to express endogenous IL-7 at some stage during development. These transgenic lines provide novel genetic tools to identify IL-7 producing cells in various tissues and to manipulate gene expression selectively in IL-7 expressing cells. genesis 47:281287, 2009. (C) 2009 Wiley-Liss, Inc.
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