期刊
GENESIS
卷 47, 期 3, 页码 142-154出版社
WILEY
DOI: 10.1002/dvg.20470
关键词
EC-SOD; TRE promoter; CamKII-tTA; LAP-tTA; transgenic mouse model
资金
- National Institutes of Health [AG24400, NS46051]
- Palo Alto Institute for Research and Education (PAIRE)
- VA Palo Alto Health Care System
The extracellular isoform of superoxide dismutase (EC-SOD, Sod3) plays a protective role against various diseases and injuries mediated by oxidative stress. To investigate the pathophysiological roles of EC-SOD, we generated tetracycline-inducible Sod3 transgenic mice and directed the tissue-specific expression of transgenes by crossing Sod3 transgenic mice with tissue-specific transactivator transgenics. Double transgenic mice with liver-specific expression of Sod3 showed increased EC-SOD levels predominantly in the plasma as the circulating form, whereas double transgenic mice with neuronal-specific expression expressed higher levels of EC-SOD in hippocampus and cortex with intact EC-SOD as the dominant form. EC-SOD protein levels also correlated well with increased SOD activities in double transgenic mice. In addition to enabling tissue-specific expression, the transgene expression can be quickly turned on and off by doxycycline supplementation in the mouse chow. This mouse model, thus, provides the flexibility for on-off control of transgene expression in multiple target tissues. genesis 47:142-154, 2009. (c) 2009 Wiley-Liss, Inc.
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