期刊
GENESIS
卷 46, 期 12, 页码 738-742出版社
WILEY
DOI: 10.1002/dvg.20437
关键词
Cre recombinase; Stra8 promoter; spermatogonia; spermatocytes; Z/EG
资金
- NICHD NIH HHS [U54 HD042454, R01 HD027215, K99 HD055330-02, U54 HD042454-08, K99 HD055330, R01 HD027215-19] Funding Source: Medline
We have generated a transgenic mouse line, Tg(Stra8-cre)1Reb (Stra8-cre), which expresses Improved Cre recombinase under the control of a 1.4 Kb promoter region of the germ cell-specific stimulated by retinoic acid gene 8 (Stra8). cre is expressed only in males beginning at postnatal day (P)3 in early-stage spermatogonia and is detected through preleptotenestage spermatocytes. To further define when cre becomes active, we crossed Stra8-cre males with Tg(ACTB-Bgeo/GFP)21Lbe (Z/EG) reporter females and compared the expression of enhanced green fluorescent protein (EGFP) with the protein encoded by the zinc finger and BTB domain containing 16 (Zbtb16) gene, PLZF-a marker for undifferentiated spermatogonia. Co-expression of EGFP is observed in the majority of PLZF+ cells. We also tested recombination efficiency by mating Stra8-cre;Z/EG males and females with wild-type mice and examining EGFP expression in the offspring. Recombination is detected in >95% of Z/EG+ pups born to Stra8-cre;Z/EG fathers but in none of the offspring born to transgenic mothers, a verification that cre is not functional in females. The postnatal, premeiotic, male germ cell-specific activity of Stra8-cre makes this mouse line a unique resource to study testicular germ cell development. genesis 46:738-742, 2008. (C) 2008 Wiley-Liss, Inc.
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