期刊
GENESIS
卷 46, 期 4, 页码 200-204出版社
WILEY-LISS
DOI: 10.1002/dvg.20384
关键词
Pax3; reporter; cardinal vein; GFP; beta-galactosidase; endothelial cell
资金
- NHLBI NIH HHS [R01-HL62974, K08-HL086633, 1P01HL075215, K08 HL086633, P01 HL075215-01A1, R01 HL062974, P01 HL075215-02, P01 HL075215] Funding Source: Medline
- NICHD NIH HHS [K12 HD000850, K12-HD00850] Funding Source: Medline
A new Cre-reporter strain of mouse has been developed that expresses a fusion protein derived from the lacZ gene fused to GFP with a nuclear localization signal. This construct is expressed from the ROSA26 locus upon Cre-mediated recombination that removes a IoxP-flanked PGK-neo cassette, thus allowing for detection of Cre activity in all tissues. This reporter strain, which is similar to prior R26R and R26EGFP strains, has certain advantages related to the nuclear expression and the combined expression of both beta-galactosidase and GFP activities. We show that the use of this newly developed reporter line allows for enhanced resolution, detection and co-localization. Thus, we report a previously unrecognized subset of venous endothelial cells derived from Pax3 expressing precursors.
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