期刊
GENES TO CELLS
卷 16, 期 12, 页码 1190-1199出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2443.2011.01561.x
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [22370066]
- Institute for Fermentation, Osaka, Japan
- Yamagata Prefectural Government and Tsuruoka City in Japan
- Grants-in-Aid for Scientific Research [22370066] Funding Source: KAKEN
We discovered that the PF1549 gene in Pyrococcus furiosus encodes a very heat-stable RNA 3'-terminal phosphate cyclase (Pf-Rtc). Although all previously reported Rtc proteins are ATP-dependent enzymes, we found that Pf-Rtc requires GTP for its cyclase activity at 95 degrees C. Low-level activation of the enzyme was also observed in the presence of dGTP but not other dNTPs, indicating that the guanine base is very important for Pf-Rtc activity. We analyzed a series of GTP analogues and found that the conversion from GTP to GMP is important for Pf-Rtc activity and that an excess of GMP inhibits this activity. Gel-shift analysis clearly showed that the RNA-binding activity of Pf-Rtc is totally dependent on the linear form of the 3'-terminal phosphate, with an apparent Kd value of 20 nm at 95 degrees C. Furthermore, we found that Pf-Rtc may contribute to GTP-dependent RNA ligation activity through the PF0027 protein (a 2'-5' RNA ligase-like protein in P. furiosus). The possible roles of Pf-Rtc and the importance of terminal phosphate structures in RNA are discussed.
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