期刊
GENES BRAIN AND BEHAVIOR
卷 13, 期 2, 页码 179-194出版社
WILEY
DOI: 10.1111/gbb.12108
关键词
Acid-sensing ion channel; ASIC1; ASIC2; brain; carbon dioxide; fear conditioning; immunocytochemistry
资金
- Fondazione Cariplo grant [2009-2528]
- FISM - Fondazione Italiana Sclerosi Multipla grant [2009/R/2]
- Iowa Cardiovascular Interdisciplinary Research Fellowship [HL07121]
- Department of Veterans Affairs
- National Institutes of Mental Health [1R01MH085724-01]
- National Institute of Heart Lung and Blood [5 R01 HL113863-01]
- McKnight Neuroscience of Brain Disorders Award
- National Institutes of Health (NIH) Clinical and Translational Science Award (CTSA) program [2 UL1 TR000442-06]
Acid-sensing ion channels (ASICs) generate H+-gated Na+ currents that contribute to neuronal function and animal behavior. Like ASIC1, ASIC2 subunits are expressed in the brain and multimerize with ASIC1 to influence acid-evoked currents and facilitate ASIC1 localization to dendritic spines. To better understand how ASIC2 contributes to brain function, we localized the protein and tested the behavioral consequences of ASIC2 gene disruption. For comparison, we also localized ASIC1 and studied ASIC1(-/-) mice. ASIC2 was prominently expressed in areas of high synaptic density, and with a few exceptions, ASIC1 and ASIC2 localization exhibited substantial overlap. Loss of ASIC1 or ASIC2 decreased freezing behavior in contextual and auditory cue fear conditioning assays, in response to predator odor and in response to CO2 inhalation. In addition, loss of ASIC1 or ASIC2 increased activity in a forced swim assay. These data suggest that ASIC2, like ASIC1, plays a key role in determining the defensive response to aversive stimuli. They also raise the question of whether gene variations in both ASIC1 and ASIC2 might affect fear and panic in humans.
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