期刊
GENES AND IMMUNITY
卷 10, 期 2, 页码 125-131出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/gene.2008.87
关键词
interferon; antiviral; cytokines; protein refolding
资金
- Novo Nordisk Foundation
- Danish Medical Research Council [22-04-0704, 271-05-0632]
- Lundbeck Foundation
Type III interferon (IFN) or IFN-lambda is a recently discovered family of IFNs that signal through the same downstream transcription factors as type I IFN but use a separate receptor complex composed of the IL-10R2 and the unique IFN-lambda R1 receptor chains. We have established a simple and efficient expression system to produce highly pure and active IFN-lambda of the three human IFN-lambda isoforms (IFN-lambda 1, -lambda 2 and -lambda 3) and used this to compare the biological activity of the different IFN-lambda subtypes. Surprisingly, we found IFN-lambda 3 to possess the highest specific activity of the human IFN-lambda subtypes, exhibiting a twofold higher activity than IFN-lambda 1 and a 16-fold higher activity than IFN-lambda 2. Furthermore, in comparison with the commercially available preparations of IFN-lambda 1 and -lambda 2, we found our IFN-lambda preparation to be superior in activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据