期刊
GENES & DEVELOPMENT
卷 28, 期 21, 页码 2370-2380出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.250993.114
关键词
mRNA 3 ' processing; cleavage and polyadenylation; polyadenylation signal
资金
- National Institutes of Health (NIH) [GM090056]
- American Cancer Society [RSG-12-186]
- University of California at Irvine
- Marie Curie Initial Training Network RNPnet [289007]
- National Center for Research Resources [5P41RR011823]
- National Institute of General Medical Sciences [8 P41 GM103533]
- NIH [GM28983]
- MRC [MC_U105185858] Funding Source: UKRI
- Medical Research Council [MC_U105185858] Funding Source: researchfish
AAUAAA is the most highly conserved motif in eukaryotic mRNA polyadenylation sites and, in mammals, is specifically recognized by the multisubunit CPSF (cleavage and polyadenylation specificity factor) complex. Despite its critical functions in mRNA 3' end formation, the molecular basis for CPSF AAUAAA interaction remains poorly defined. The CPSF subunit CPSF160 has been implicated in AAUAAA recognition, but direct evidence has been lacking. Using in vitro and in vivo assays, we unexpectedly found that CPSF subunits CPSF30 and Wdr33 directly contact AAUAAA. Importantly, the CPSF30 RNA interaction is essential for mRNA 3' processing and is primarily mediated by its zinc fingers 2 and 3, which are specifically targeted by the influenza protein NS1A to suppress host mRNA 3' processing. Our data suggest that AAUAAA recognition in mammalian mRNA 3' processing is more complex than previously thought and involves multiple protein RNA interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据