期刊
GENES & DEVELOPMENT
卷 28, 期 14, 页码 1550-1555出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.245662.114
关键词
RNA polymerase II; core promoter; TRF2; TCT motif; ribosomal protein genes
资金
- University of California at SanDiego Molecular Biology/Cancer Center Fellowship
- National Institutes of Health [1DP2OD004561-01, R01 GM041249]
The TCT core promoter element is present in most ribosomal protein (RP) genes in Drosophila and humans. Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP genes. In cells, TCT-dependent transcription, but not TATA-dependent transcription, increases or decreases upon overexpression or depletion of TRF2. In vitro, purified TRF2 activates TCT but not TATA promoters. ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep sequencing) experiments revealed the preferential localization of TRF2 at TCT versus TATA promoters. Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems.
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