期刊
GENES & DEVELOPMENT
卷 27, 期 5, 页码 541-551出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.209635.112
关键词
mRNA decay; single-RNA FISH; mRNA quality control
资金
- Japan Society for the Promotion of Science
- NIH [R01 GM84364, GM86217, R01GM59614]
Nonsense-mediated mRNA decay (NMD) is a quality control mechanism responsible for surveying mRNAs during translation and degrading those that harbor a premature termination codon (PTC). Currently the intracellular spatial location of NMD and the kinetics of its decay step in mammalian cells are under debate. To address these issues, we used single-RNA fluorescent in situ hybridization (FISH) and measured the NMD of PTC-containing beta-globin mRNA in intact single cells after the induction of beta-globin gene transcription. This approach preserves temporal and spatial information of the NMD process, both of which would be lost in an ensemble study. We determined that decay of the majority of PTC-containing beta-globin mRNA occurs soon after its export into the cytoplasm, with a half-life of <1 min; the remainder is degraded with a half-life of >12h, similar to the half-life of normal PTC-free beta-globin mRNA, indicating that it had evaded NMD. Importantly, NMD does not occur within the nucleoplasm, thus countering the long-debated idea of nuclear degradation of PTC-containing transcripts. We provide a spatial and temporal model for the biphasic decay of NMD targets.
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