期刊
GENES & DEVELOPMENT
卷 26, 期 1, 页码 11-24出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.179804.111
关键词
chromosome conformation capture; functional genomics; genome structure; nuclear organization
资金
- Dutch Scientific Organization (NWO) [700.10.402, 91204082, 935170621]
- InteGeR FP7 Marie Curie ITN [PITN-GA-2007-214902]
- European Research Council [209700, 4C]
- European Research Council (ERC) [209700] Funding Source: European Research Council (ERC)
Over the past 10 years, the development of chromosome conformation capture (3C) technology and the subsequent genomic variants thereof have enabled the analysis of nuclear organization at an unprecedented resolution and throughput. The technology relies on the original and, in hindsight, remarkably simple idea that digestion and religation of fixed chromatin in cells, followed by the quantification of ligation junctions, allows for the determination of DNA contact frequencies and insight into chromosome topology. Here we evaluate and compare the current 3C-based methods (including 4C [chromosome conformation capture-on-chip], 5C [chromosome conformation capture carbon copy], HiC, and ChIA-PET), summarize their contribution to our current understanding of genome structure, and discuss how shape influences genome function.
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