期刊
GENES & DEVELOPMENT
卷 25, 期 12, 页码 1205-1231出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.2050011
关键词
FRET; optical tweezers; AFM; single molecule; translation
资金
- NIH-NIGMS [R01 GM10840, R01 GM084288]
- Burroughs Wellcome Fund [CABS 1004856]
- NSF [MCB 0644262]
- American Cancer Society [GMC-117152]
The last 15 years have witnessed the development of tools that allow the observation and manipulation of single molecules. The rapidly expanding application of these technologies for investigating biological systems of ever-increasing complexity is revolutionizing our ability to probe the mechanisms of biological reactions. Here, we compare the mechanistic information available from single-molecule experiments with the information typically obtained from ensemble studies and show how these two experimental approaches interface with each other. We next present a basic overview of the toolkit for observing and manipulating biology one molecule at a time. We close by presenting a case study demonstrating the impact that single-molecule approaches have had on our understanding of one of life's most fundamental biochemical reactions: the translation of a messenger RNA into its encoded protein by the ribosome.
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