期刊
GENES & DEVELOPMENT
卷 25, 期 1, 页码 64-76出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1985511
关键词
ADP ribosylation toxin; Agrobacterium protein 6b; crystal structure; miRNA machinery; miRNA phenotype
资金
- Singapore Ministry of Education [T208A3124]
- Temasek Life Sciences Laboratory
- NIH [GM44640]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R56GM044640, R01GM044640] Funding Source: NIH RePORTER
The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 angstrom, Agrobacterium vitis AB6b at 1.65 angstrom, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 angstrom. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据