期刊
GENES & DEVELOPMENT
卷 25, 期 2, 页码 119-124出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1998711
关键词
erythropoiesis; enucleation; RNA-seq; miR-191; Riok3; Mxi1
资金
- Singapore-Massachusetts Institute of Technology Alliance
- SMA [C-382-641-001-091]
- NIH [DK047636, AI054973, DK047618, DK068348, 5P01 HL066105]
Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1.
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