期刊
GENES & DEVELOPMENT
卷 23, 期 22, 页码 2610-2624出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1823209
关键词
RNA polymerase II transcription; gene loops; transcriptional memory; S. cerevisiae; nuclear pore complex
资金
- Medical Research Council [G9826944] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Medical Research Council [G9826944] Funding Source: researchfish
- MRC [G9826944] Funding Source: UKRI
Inducible genes in yeast retain a memory'' of recent transcriptional activity during periods of short-term repression, allowing them to be reactivated faster when reinduced. This confers a rapid and versatile gene expression response to the environment. We demonstrate that this memory mechanism is associated with gene loop interactions between the promoter and 39 end of the responsive genes HXK1 and GAL1::FMP27. The maintenance of these memory gene loops ( MGLs) during intervening periods of transcriptional repression is required for faster RNA polymerase II (Pol II) recruitment to the genes upon reinduction, thereby facilitating faster mRNA accumulation. Notably, a sua7-1 mutant or the endogenous INO1 gene that lacks this MGL does not display such faster reinduction. Furthermore, these MGLs interact with the nuclear pore complex through association with myosin-like protein 1 (Mlp1). An mlp1 Delta strain does not maintain MGLs, and concomitantly loses transcriptional memory. We predict that gene loop conformations enhance gene expression by facilitating rapid transcriptional response to changing environmental conditions.
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