期刊
GENES & DEVELOPMENT
卷 22, 期 9, 页码 1141-1146出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.473408
关键词
polyadenylation; epigenetics; imprinting; Mcts2
资金
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
- Medical Research Council [MC_U142636336, MC_UP_1502/1] Funding Source: Medline
- Wellcome Trust [071002] Funding Source: Medline
- Medical Research Council [MC_U142636336, MC_UP_1502/1] Funding Source: researchfish
- MRC [MC_UP_1502/1, MC_U142636336] Funding Source: UKRI
Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in co-transcriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites.
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