期刊
GENES & DEVELOPMENT
卷 22, 期 5, 页码 575-580出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1606308
关键词
Nanog; Oct4; chromatin; reprogramming; self renewal
资金
- Howard Hughes Medical Institute Funding Source: Medline
- NHGRI NIH HHS [U01HG003161] Funding Source: Medline
- NIGMS NIH HHS [R01GM071923] Funding Source: Medline
Embryonic stem (ES) cells offer insight into early developmental fate decisions, and their controlled differentiation may yield vast regenerative potential. The molecular determinants supporting ES cell self-renewal are incompletely understood. The homeodomain proteins Nanog and Oct4 are essential for mouse ES cell self-renewal. Using a high-throughput approach, we discovered DNaseI hypersensitive sites and potential regulatory elements along a 160-kb region of the genome that includes GDF3, Dppa3, and Nanog. We analyzed gene expression, chromatin occupancy, and higher-order chromatin structure throughout this gene locus and found that expression of the reprogramming factor Oct4 is required to maintain its integrity.
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