期刊
GENES & DEVELOPMENT
卷 22, 期 6, 页码 728-733出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1641808
关键词
zebrafish; fin; regeneration; miR-133; Fgf; Mps1
资金
- NHLBI NIH HHS [2 T-32 HL007101-29, T32 HL007101, R01 HL081674] Funding Source: Medline
- NIGMS NIH HHS [R01 GM074057-03, R01 GM074057-02, R01 GM074057] Funding Source: Medline
Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration.
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