期刊
GENES & DEVELOPMENT
卷 22, 期 8, 页码 1069-1081出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.463708
关键词
ribosome synthesis; rRNA synthesis; RNA polymerase I; exonuclease; transcription termination
资金
- Biotechnology and Biological Sciences Research Council [BB/F010656/1] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/F010656/1] Funding Source: researchfish
- BBSRC [BB/F010656/1] Funding Source: UKRI
During transcription termination by RNA polymerase II on protein-coding genes, the nuclear 5' exonuclease Rat1/Xrn2 degrades the nascent transcript downstream from the polyadenylation site and torpedoes the polymerase. We report that the activity of Rat1 is also required for efficient termination by RNA polymerase I (Pol I) on the rDNA. In strains lacking catalytically active Rat1 or its cofactor Rai1, Pol I reads through the major, Reb1-dependent terminator (T1) but stops downstream at the fail-safe terminator (T2) and replication fork barrier (RFB). The absence of both Rat1 and the RFB-binding protein Fob1 increased Pol I read-through of T2 and the RFB. We propose that cotranscriptional cleavage of the pre-rRNA by the endonuclease Rnt1 generates a loading site for the Rat1/Rai1 complex, which then degrades the nascent transcript. When Rat1 catches Pol I, which is predicted to be paused at T1, transcription is terminated.
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