期刊
GENES & DEVELOPMENT
卷 22, 期 9, 页码 1231-1243出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1643308
关键词
actomyosin; angiogenesis; endothelial cells; extracellular matrix; fibronectin
资金
- Howard Hughes Medical Institute [r21 eb006890] Funding Source: Medline
- NCI NIH HHS [R01 CA088308, CA88308] Funding Source: Medline
- NHLBI NIH HHS [HL21644, R01 HL021644] Funding Source: Medline
- NIBIB NIH HHS [R21 EB006890] Funding Source: Medline
During vasculogenesis and angiogenesis, endothelial cell responses to growth factors are modulated by the compositional and mechanical properties of a surrounding three-dimensional (3D) extracellular matrix (ECM) that is dominated by either cross-linked fibrin or type I collagen. While 3D-embedded endothelial cells establish adhesive interactions with surrounding ligands to optimally respond to soluble or matrix-bound agonists, the manner in which a randomly ordered ECM with diverse physico- mechanical properties is remodeled to support blood vessel formation has remained undefined. Herein, we demonstrate that endothelial cells initiate neovascularization by unfolding soluble fibronectin (Fn) and depositing a pericellular network of fibrils that serve to support cytoskeletal organization, actomyosin-dependent tension, and the viscoelastic properties of the embedded cells in a 3D-specific fashion. These results advance a new model wherein Fn polymerization serves as a structural scaffolding that displays adhesive ligands on a mechanically ideal substratum for promoting neovessel development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据