期刊
GENES & DEVELOPMENT
卷 22, 期 17, 页码 2342-2346出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1696108
关键词
in vivo quantitation; intron; negative feedback; oscillations; synthetic biology; transcription elongation
资金
- National Institutes of Health
- Minesterio de Educacion y Ciencia of Spain
Introns may affect gene expression by increasing the time required to transcribe the gene. One way for extended transcription times to affect the behavior of a gene expression program is through a negative feedback loop. Here, we show that a logically engineered negative feedback loop in animal cells produces expression pulses, which have a broad time distribution that increases with intron length. These results in combination with mathematical models provide insight into what may produce the intron-dependent pulse distributions. We conclude that the long production time required for large intron-containing genes is significant for the behavior of gene expression programs.
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