期刊
GENES & DEVELOPMENT
卷 22, 期 20, 页码 2767-2772出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.503108
关键词
RecQ helicases; DNA resection; DNA repair; DNA damage checkpoint; Exonuclease 1; ATR
资金
- Cancer Research UK
- European Union
A key cellular response to DNA double-strand breaks ( DSBs) is 5 '-to-3 ' DSB resection by nucleases to generate regions of ssDNA that then trigger cell cycle checkpoint signaling and DSB repair by homologous recombination (HR). Here, we reveal that in the absence of exonuclease Exo1 activity, deletion or mutation of the Saccharomyces cerevisiae RecQ-family helicase, Sgs1, causes pronounced hypersensitivity to DSB-inducing agents. Moreover, we establish that this reflects severely compromised DSB resection, deficient DNA damage signaling, and strongly impaired HR-mediated repair. Furthermore, we show that the mammalian Sgs1 ortholog, BLM - whose deficiency causes cancer predisposition and infertility in people - also functions in parallel with Exo1 to promote DSB resection, DSB signaling and resistance to DSB-generating agents. Collectively, these data establish evolutionarily conserved roles for the BLM and Sgs1 helicases in DSB processing, signaling, and repair.
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