4.2 Article

Identification and characterization of a motilin-like peptide and its receptor in teleost

期刊

GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 186, 期 -, 页码 85-93

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2013.02.018

关键词

Motilin; Ghrelin; Motilin receptor; Zebrafish

资金

  1. Special Fund for Agro-Scientific Research in Public Interest [200903046]
  2. National 863 Program of China [2012AA10A407]
  3. Hong Kong Research Grants Council [462409]
  4. National Science Foundation of China [31072196, 31101886, 31261160493]
  5. Guangdong Provincial Natural Science Foundation [S20110400016030]
  6. China Postdoctoral Science Foundation [20100480815]
  7. Fundamental Research Funds for the Central Universities [101gpy21, 131gpy17]

向作者/读者索取更多资源

Although putative motilin receptor sequences have been reported in teleost, there is no proof for the existence of the motilin gene in teleost. In this study, we have identified a motilin-like gene in the genome of several fish species and cloned its cDNA sequence from zebrafish. The zebrafish motilin-like precursor shares very low amino acid (aa) identities with the previously reported motilin precursors. Processing of the zebrafish motilin-like precursor may generate a 17-aa C-terminal amidated mature peptide, the motilin-like peptide (motilin-LP). A putative zebrafish motilin receptor (MLNR) was also identified in zebrafish. In cultured eukaryotic cells transfected with the zebrafish MLNR, zebrafish motilin-LP could enhance both CRE-driven and SRE-driven promoter activities. Tissue distribution studies indicated that the zebrafish motilin-like gene is mainly expressed in the intestine and liver while the zebrafish MLNR gene is highly expressed in brain regions, suggesting that motilin-LP behaves like other gut hormones to regulate brain functions. These data suggest that the presence of a unique motilin/MNLR system in teleost. (C) 2013 Elsevier Inc. All rights reserved.

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