Female-enriched expression of ERα during gonad differentiation of the urodele amphibian Pleurodeles waltl

In the amphibian Pleurodeles waltl, estradiol treatment of genetically male larvae (ZZ) induces male-to-female sex reversal whereas heat treatment of genetically female larvae (ZW) inhibits estradiol synthesis and leads to female-to-male sex reversal. No data are available on estrogen receptors in this species. In the present study, we have isolated a unique full-length pwER alpha cDNA and its 5'-flanking region whose promoter activity was confirmed by transfection assays. RT-PCR studies performed in adult animals using ER alpha-specific primers, revealed that pwER alpha mRNA was present mainly in reproductive tissues: gonads, fat body and oviduct. PwER alpha transcript was also detected in liver, suggesting its implication in vitellogenesis control as in numerous oviparous species. The level of pwER alpha transcripts was also studied during gonad differentiation by quantitative real-time PCR. At stage 54(30d) pwER alpha expression in gonads of ZW larvae was significantly higher than in ZZ ones. This sex-specific discrimination was confirmed when gonad-mesonephros-interrenal complexes (GMI), taken at the same stage, were subjected to whole mount in situ hybridization. In comparison, the female-enriched expression of P450 aromatase, which was studied as a control of ovary differentiation, was observed earlier (stage 54). In ZW larvae reared at 32 degrees C, a condition leading to sex reversal, pwER alpha mRNA level at stage 54(30d) was lower than in control females. Taken together, these results showing a female-enriched and thermosensitive expression of pwER alpha suggest an important role for this receptor in gonad differentiation of the urodele amphibian Pleurodeles waltl. (C) 2008 Elsevier Inc. All rights reserved.