期刊
GENE THERAPY
卷 17, 期 2, 页码 190-201出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/gt.2009.142
关键词
adenovirus; decorin; extracellular matrix; viral spreading
类别
资金
- Ministry of Commerce Industry and Energy [10030051]
- Korea Research Foundation [KRF-2005-042C00148]
- Korea Science and Engineering Foundation (KOSEF) [R01-2006-000-10084-0, R15-2004 024-02001-0, M10416130002-04N1613-00210]
- National Nuclear Technology Program [2007-00299]
- National Core Research Center for Nanomedical Technology
- Medical Science, Yonsei University College of Medicine, Seoul, South Korea
- National Research Foundation of Korea [R01-2006-000-10084-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The pressing challenge for contemporary gene therapy is to deliver enough therapeutic genes to enough cancer cells in vivo. With the aim of improving viral distribution and tumor penetration, we explored the use of decorin to enhance viral spreading and tumor tissue penetration. We generated decorin-expressing replication-incompetent (dl-LacZ-DCNG, dl-LacZ-DCNQ and dl-LacZ-DCNK) and replication-competent (Ad-Delta E1B-DCNG, Ad-Delta E1B-DCNQ and Ad-Delta E1B-DCNK) adenoviruses (Ads). Point mutants of decorin gene (DCNG), DCNK and DCNQ, have a negative and moderate binding affinity to type-I collagen fibril, respectively. In both tumor spheroids and established solid tumors in vivo, tissue penetration potency of dl-LacZ-DCNG was greatly enhanced than those of dl-LacZ, dl-LacZ-DCNQ and dl-LacZ-DCNK, and this enhanced tissue penetration effect derived from decorin-expressing Ad was dependent on the binding affinity of decorin to collagen fibril. Expression of DCNG enhanced viral spread of replicating Ad, leading to improved tumor reduction and survival benefit. Moreover, the tumoricidal effects of Ad-Delta E1B-DCNQ and Ad-Delta E1B-DCNK were lessened, as the binding affinity to collagen was decreased, showing that the increased cancer cell cytotoxicity was driven by the action of decorin on extracellular matrix (ECM). Furthermore, Ad-Delta E1B-DCNG substantially decreased ECM components within the tumor tissue. Finally, intratumoral injection of Ad-Delta E1B-DCNG in primary tumor site greatly reduced the formation of B16BL6 melanoma cell pulmonary metastases in mice. Taken together, these data show the utility of decorin as a dispersion agent and highlight its utility and potential in improving the efficacy of replicating Ad-mediated cancer gene therapy. Gene Therapy (2010) 17, 190-201; doi: 10.1038/gt.2009.142; published online 12 November 2009
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