期刊
GENE EXPRESSION PATTERNS
卷 11, 期 1-2, 页码 118-121出版社
ELSEVIER
DOI: 10.1016/j.gep.2010.10.002
关键词
Zebrafish; Pronephros; Kidney; Promoter; Development; Transgenic; Glutamyl aminopeptidase; Enpep; Aminopeptidase A
资金
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK054942] Funding Source: NIH RePORTER
- NIDDK NIH HHS [R01 DK054942-01A1, R01 DK054942] Funding Source: Medline
In recent years the zebrafish has become a popular model system to study organ development and disease. To facilitate these studies, genetic tools are required which allow to modify and manipulate gene expression in organs of interest. Here we describe a zebrafish 2 kb glutamyl aminopeptidase (enpep) promoter fragment, and show that it can drive gene expression specifically in the kidney during early and late development. We established a stable transgenic line using this promoter fragment that has specific GFP expression in pronephric ducts and tubules starting at 20 h post-fertilization. (c) 2010 Elsevier B.V. All rights reserved.
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