4.6 Article

Characteristic of PGDS potential regulation role on spermatogenesis in the Chinese mitten crab Eriocheir sinensis

期刊

GENE
卷 543, 期 2, 页码 244-252

出版社

ELSEVIER
DOI: 10.1016/j.gene.2014.04.010

关键词

PGDS; Spermatogenesis; Eriocheir sinensis

资金

  1. Public Welfare Agricultural Scientific Research [201203065]
  2. Basic Research Funds from Freshwater Fisheries Research Center [2013JBFR02]
  3. National Infrastructure of Fishery Germplasm Resources [2014DKA3047003]

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Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH2 to produce PGD2 in the presence of sulfhydryl compounds. In this study, a full length PGDS,gene comprising 1250 nucleotides from the Chinese mitten crab Eriocheir sinensis (Es-PGDS) was characterized, with a 615 bp open reading frame encoding 204 amino acid residues. Its deduced peptide has high homology with other species' PGDS protein. The Es-PGDS mRNA expression was tissue-related, with the highest expression observed in the hepatopancreas, accessory sex gland, testis and ovaries. We also detected the different stages of tissue expression and the enzyme activity for Es-PGDS in the testis and male crab hepatopancreas. The different expression patterns and its corresponding enzyme activity level indicated that PGDS is involving in the regulation of reproductive action during the period of rapid development in E. sinensis. Furthermore our research could arouse a heat debate on the PGDS reproductive function in invertebrate and further study will be needed to determine the molecular mechanism(s) linking PGDS functions to spermatogenesis and ontogenesis if this gene is to be exploited as a molecular biomarker in further studies of development. (C) 2014 Elsevier B.V. All rights reserved.

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