期刊
GENE
卷 543, 期 1, 页码 15-21出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2014.04.016
关键词
Heart; Ischemia; Long noncoding RNA; Reperfusion
资金
- Natural Science Foundation of China [81202187]
Long noncoding RNAs (IncRNAs) play important regulatory roles in cellular physiology. The contributions of IncRNAs to ischemic heart disease remain largely unknown. The aim of this study was to investigate the profile of myocardial IncRNAs and their potential roles at early stage of reperfusion. IncRNAs and mRNAs were profiled by microarray and the expression of some highly-dysregulated IncRNAs was further validated using polymerase chain reaction. Our results revealed that 64 IncRNAs were up-regulated and 87 down-regulated, while 50 mRNAs were up-regulated and 60 down-regulated in infarct region at all reperfusion sampled. Gene ontology analysis indicated that dysregulated transcripts were associated with immune response, spermine catabolic process, taxis, chemotaxis, polyamine catabolic process, spermine metabolic process, chemokine activity and chemokine receptor binding. Target gene-related pathway analysis showed significant changes in cytokine-cytokine receptor interaction, the chemokine signaling pathway and nucleotide oligomerization domain (NOD)-like receptor signaling pathway which have a close relationship with myocardial ischemia/reperfusion injury (MI/RI). Besides, a gene co-expression network was constructed to identify correlated targets of 10 highly-dysregulated IncRNAs. These IncRNAs may play their roles by this network in post-ischemic heart. Such results provide a foundation for understanding the roles and mechanisms of myocardial lncRNAs at early stage of reperfusion. (C) 2014 Elsevier rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据