期刊
GENE
卷 513, 期 1, 页码 118-127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2012.10.058
关键词
Cold-tolerance gene of tree; Galactinol synthase gene of Ammopiptanthus mongolicus (AmGS); Transgenic ligneous plant
资金
- Science and Technology Innovation Program of Chinese Academy of Sciences [057310A151]
- Key Research Project in Science and Technology of Shandong Provence [2010GG10009008]
- State Key Project in Development of New Plant Varieties by Gene Transformation [2009ZX08009-100B]
A cold induced galactinol synthase gene (AmGS) and its promoter sequence were identified and cloned from the cold-tolerant tree Ammopiptanthus mongolicus by using cDNA-AFLP, RACE-PCR and TAIL-PCR strategies combined with its expression pattern analysis after cold inducing treatment. Accession number of the AmGS gene in GenBank is DQ519361. The open reading frame (ORF) region of the AmGS gene is 987 nucleotides encoding for 328 amino acid residues and a stop codon. The genomic DNA sequence of AmGS gene contains 3 exons and 2 introns. Moreover, a variety of temporal gene expression patterns of AmGS was detected, which revealed the up-regulation of AmGS gene in stresses of cold, ABA and others. Then the AmGS gene was transformed into Photinia serrulata tree by Agrobacterium-mediated transformation, and the transgenic plants exhibited higher cold-tolerance comparing with non-transformed plants. (c) 2012 Elsevier B.V. All rights reserved.
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