期刊
GENE
卷 523, 期 2, 页码 122-125出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2013.03.140
关键词
Class-IIS endonuclease; Direct full-length polymerase chain reaction (PCR); DNA assembling; Ligation; Multiple site-directed mutagenesis
资金
- National Natural Science Foundation of China [30973878]
- Excellent Research Team Cultivation Program of Shanghai University of Traditional Chinese Medicine
An efficient DNA assembling strategy was developed here modified from Class-IS endonuclease mediated DNA splicing by directed ligation (SDL). Benefited from the full-length PCR directly using ligation products as template, this strategy required less effort and less time to obtain the assembled full-length DNA. The advantages of this strategy made it a rapid and easy-to-perform gene splicing and multiple site-directed mutagenesis approach especially practicable when more fragments need to be assembled at the same time. (C) 2013 Elsevier B.V. All rights reserved.
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