期刊
GENE
卷 524, 期 2, 页码 246-252出版社
ELSEVIER
DOI: 10.1016/j.gene.2013.04.012
关键词
Limonene synthase gene; Mentha L; Gene diversity; Molecular evolution; Genetic relationship
资金
- Natural Science Foundation of Jiangsu Province [BK2009330, BK2010475]
- Agricultural Scientific and Technological Independent Innovation Foundation of Jiangsu Province [CX(11)1021]
The genus Mentha comprises a group of aromatic plants with worldwide distribution. Because of frequent interspecific hybridization, the genetic relationships within the genus are not clearly understood. Limonene synthase, which catalyses the first committed step in the essential oil monoterpene biosynthetic pathway, is considered to be a possible rate limiting enzyme. With the homology-based cloning method, primers were designed according to cDNA sequence to amplify full-length DNA sequences in 13 Mentha samples from five species, using Perilla as an outgroup. Analyses of gene structure, length variation, GC-content, Ts/Tv ratio and evolutionary diversity were carried out. Consensus phylogenetic trees were obtained using maximum likelihood, neighbor-joining, and maximum parsimony, respectively, based on the full-length genomic DNA sequences, complete ORF coding sequences and predicted amino acid sequences. The results presented here based on the sequence of MhLS provide the first credibly supported genetic relationships for Mentha, which enables a basis for further mint taxonomy, cultivation and breeding. (C) 2013 Elsevier B.V. All rights reserved.
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