期刊
GENE
卷 499, 期 1, 页码 76-80出版社
ELSEVIER
DOI: 10.1016/j.gene.2012.02.016
关键词
Araliaceae; DNA barcoding; ITS2
资金
- National Natural Science Foundation of China [81102746]
- Beijing Natural Science Foundation [5113033]
- Scientific Research Foundation of the State Human Resource Ministry and the Education Ministry for Returned Chinese Scholars
- Peking Union Medical College
- Youth Foundation of Peking Union Medical College
- Research Fund for the Doctoral Program of Higher Education [20111106120028]
- Major Drug Discovery major science and technology research 12nd Five-Year Plan [2012ZX09301-002-001]
- China Medical Board of New York [A2009001]
An effective DNA marker in authentication of the family Araliaceae was screened out of the five DNA regions (matK, rbcL, ITS2, psbA-trnH and ycf5). In the present study, 1113 sequences of 276 species from 23 genera (Araliaceae) were collected from DNA sequencing and GenBank, in which 16 specimens were from 5 provinces in China and Japan. All of the sequences were assessed in the success rates of PCR amplifications, intra- and inter-specific divergence. DNA barcoding gaps and efficiency of identification. Compared with other markers, ITS2 showed superiority in species discrimination with an accurate identification of 85.23% and 97.29% at the species and genus levels, respectively, in plant samples from the 589 sequences derived from Araliaceae. Consequently, as one of the most popular phylogenetic markers, our study indicated that ITS2 was a powerful barcode for Araliaceae identification. (C) 2012 Elsevier B.V. All rights reserved.
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