期刊
JOURNAL OF VASCULAR RESEARCH
卷 52, 期 1, 页码 62-69出版社
KARGER
DOI: 10.1159/000382129
关键词
Angiogenesis; Endothelial progenitor cells; Therapeutic neovascularization; Vascular endothelial growth factor; Stromal cell-derived factor
资金
- National Institutes of Health [RO1 HL069957]
- Wyss Institute for Biologically Inspired Engineering at Harvard University
Endothelial progenitor cells are being broadly explored for the treatment of ischemic cardiovascular diseases, but their response to molecules commonly used to promote the growth of new blood vessels has not been fully characterized. In this study, angiogenic sprout formation in a 3-dimensional, in vitro model by one type of endothelial progenitor, outgrowth endothelial cells (OECs), was characterized in response to exposure to stromal cell-derived factor (SDF) and vascular endothelial growth factor (VEGF) and then compared to mature endothelial cells. Exposure to SDF alone did not increase angiogenic sprouting in comparison to control media, while a combination of VEGF and SDF demonstrated greater potency than VEGF alone for all cell types. Together, VEGF and SDF reduced the sprout initiation time and maintained sprouting levels over time. In direct competition with mature endothelial cells, OECs preferentially localized to the tip cell position, suggesting an enhanced sprouting potential. Overall, these results reveal the impact of the combination of VEGF and SDF on endothelial cell sprouting, and support the enhanced potential of OECs, as opposed to mature endothelial cells, for treating ischemic diseases. (C) 2015 S. Karger AG, Basel
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