Endocytoscopy for the detection of microstructural features in adult patients with celiac sprue: a prospective, blinded endocytoscopy-conventional histology correlation study

Background: Endocytoscopy (EC) is a novel technique that allows magnified live inspection of the intestinal mucosa. Objective: To evaluate EC for the detection of key pathological findings in patients with celiac sprue. Design: A total of 166 EC recordings were prospectively acquired. Matched videos, images, and biopsy specimens were obtained by duodenal argon beamer labeling of the respective sites. Setting: Academic tertiary referral center. Patients: Forty patients (mean age 51.5 years, 70% women) with established (n = 32) or suspected (n = 8) celiac disease (CD). Interventions: A validated scoring system (Marsh classification) was used to assess disease activity. EC criteria were independently evaluated by 2 gastroenterologists and I pathologist. Main Outcome Measurements: The primary endpoint was to examine EC correlation with conventional CD histology. Results: Of 166 duodenal biopsy sites, 23% were classified as Marsh III (moderate to severe), 10% as Marsh I (mild), and 67% as Marsh 0 (normal). Using the 450x magnification, We found that identification of crypts was diagnostic for celiac pathology. Four criteria were significant predictors of Marsh III pathology when adjusted by multivariate analysis: low number of villi per visual field (<3; odds ratio [OR] 9.1; 95% CI, 1.3-62.0), confluence of villi (OR 37.1; 95% CI, 1.3-1021.2), irregular epithelial lining (OR 10.9; 95% CI, 2.5-46.7), and inability to delineate loop capillaries (OR 1.4.9; 95% CI, 3.3-67.0). None was a good predictor of Marsh I pathology Limitations: Single-center experience. No prospective validation of the criteria in an independent patient population. Conclusions: EC at 450x magnification accurately identifies mucosal histopathology of advanced CD, but not early morphological changes. (Gastrointest Endosc 2009;70:933-41.)