期刊
GASTROENTEROLOGY
卷 146, 期 4, 页码 995-U544出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1053/j.gastro.2014.01.006
关键词
Serotonin; Linoleic Acid; GLP-1; Lipids
资金
- French Ministry of Higher Education and Research
- Region Bourgogne
- ANR SensoFat-2 [ANR-12-BSV1-0027-01]
- National Institutes of Health (Abumrad) [DK033301, DK060022]
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV1-0027] Funding Source: Agence Nationale de la Recherche (ANR)
BACKGROUND & AIMS: It is important to increase our understanding of gustatory detection of dietary fat and its contribution to fat preference. We studied the roles of the fat taste receptors CD36 and GPR120 and their interactions via Ca2+ signaling in fungiform taste bud cells (TBC). METHODS: We measured Ca2+ signaling in human TBC, transfected with small interfering RNAs against messenger RNAs encoding CD36 and GPR120 (or control small interfering RNAs). We also studied Ca2+ signaling in TBC from CD36(-/-) mice and from wild-type lean and obese mice. Additional studies were conducted with mouse enteroendocrine cell line STC-1 that express GPR120 and stably transfected with human CD36. We measured release of serotonin and glucagon-like peptide-1 from human and mice TBC in response to CD36 and GPR120 activation. RESULTS: High concentrations of linoleic acid induced Ca2+ signaling via CD36 and GPR120 in human and mice TBC, as well as in STC-1 cells, and low concentrations induced Ca2+ signaling via only CD36. Incubation of human and mice fungiform TBC with lineoleic acid down-regulated CD36 and up-regulated GPR120 in membrane lipid rafts. Obese mice had decreased spontaneous preference for fat. Fungiform TBC from obese mice had reduced Ca2+ and serotonin responses, but increased release of glucagon-like peptide-1, along with reduced levels of CD36 and increased levels of GPR120 in lipid rafts. CONCLUSIONS: CD36 and GPR120 have nonoverlapping roles in TBC signaling during orogustatory perception of dietary lipids; these are differentially regulated by obesity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据