期刊
FUTURE MICROBIOLOGY
卷 9, 期 8, 页码 935-946出版社
FUTURE MEDICINE LTD
DOI: 10.2217/FMB.14.61
关键词
detection; foodborne pathogen; high-throughput screening; rapid
类别
资金
- US Department of Agriculture [1935-42000-035, 106037]
- Center for Food Safety Engineering at Purdue University
Foodborne pathogens pose serious public health risks. Rapid, accurate technologies to detect a low number of target cells (1 cell/25-325 g sample) and microbial toxins are in demand in order to assess product safety in hours to up to 1 day. Varied pathogen loads and the complexity of food present a major challenge. Current culture methods, while accurate, are lengthy. New methods, using brief culturing and detection kits (antibody based, nucleic acid amplification or nano/biosensors) or a culture-independent approach coupled with nucleic acid amplification, traditionally used for viruses/parasites, can be used to obtain results in hours. A strategic approach involving two-step, rapid, high-throughput screening to rule out negatives followed by a confirmatory test could accomplish product testing in 1 h to 1 day.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据