期刊
FUNGAL GENETICS AND BIOLOGY
卷 48, 期 4, 页码 430-437出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2010.12.001
关键词
Secondary metabolism; Aspergillus niger; Natural products; Genomics; Naphtho-gamma-pyrone; Polyketides
资金
- National Institute of General Medical Sciences [PO1GM084077]
- Department of Energy, Office of the Biomass Program
- US Department of Energy's Office of Science, Biological and Environmental Research [ATCC 1015]
- University of California, Lawrence Berkeley National Laboratory [DE-AC02-05CH11231]
- Los Alamos National Laboratory [DE-AC02-06NA25396]
The genome sequencing of the fungus Aspergillus niger uncovered a large cache of genes encoding enzymes thought to be involved in the production of secondary metabolites yet to be identified. Identification and structural characterization of many of these predicted secondary metabolites are hampered by their low concentration relative to the known A. niger metabolites such as the naphtho-gamma-pyrone family of polyketides. We deleted a non-reducing PKS gene in A. niger strain ATCC 11414. a daughter strain of A. niger ATCC strain 1015 whose genome was sequenced by the DOE Joint Genome Institute. This PKS encoding gene we name albA is a predicted ortholog of alb1 from Aspergillus fumigatus which is responsible for production of the naphtho-gamma-pyrone precursor for the 1,8-dihydroxynaphthalene (DHN) melanin/spore pigment. Our results show that the A. niger albA PKS is responsible for both the production of the spore pigment precursor and a family of naphtho-gamma-pyrones commonly found in significant quantity in A. niger culture extracts. The generation of an A. niger strain devoid of naphtho-gamma-pyrones will greatly facilitate the elucidation of cryptic biosynthetic pathways in this organism. (c) 2010 Elsevier Inc. All rights reserved.
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