期刊
FUNGAL GENETICS AND BIOLOGY
卷 48, 期 6, 页码 573-584出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2010.11.003
关键词
Metal homeostasis; Black truffle genome; Metal transportome; Phytochelatin synthase; Metallothionein; Mycorrhizae
资金
- Fondazione Cariparma (Parma, Italy)
- Ministry of Education, University and Research (Rome, Italy
- MIUR)
- European Network of Excellence EVOLTREE
- Region Lorraine Council
- Fondazione Cariparma
- MIUR
- European Project ENERGYPOPLAR
- Region Lorraine
Ectomycorrhizal fungi are thought to enhance mineral nutrition of their host plants and to confer increased tolerance toward toxic metals. However, a global view of metal homeostasis-related genes and pathways in these organisms is still lacking. Building upon the genome sequence of Tuber melanosporum and on transcriptome analyses, we set out to systematically identify metal homeostasis-related genes in this plant-symbiotic ascomycete. Candidate gene products (101) were subdivided into three major functional classes: (i) metal transport (58); (ii) oxidative stress defence (32); (iii) metal detoxification (11). The latter class includes a small-size metallothionein (TmelMT) that was functionally validated in yeast, and phytochelatin synthase (TmelPCS), the first enzyme of this kind to be described in filamentous ascomycetes. Recombinant TmelPCS was shown to support GSH-dependent, metal-activated phytochelatin synthesis in vitro and to afford increased Cd/Cu tolerance to metal hypersensitive yeast strains. Metal transporters, especially those related to Cu and Zn trafficking, displayed the highest expression levels in mycorrhizae, suggesting extensive translocation of both metals to root cells as well as to fungal metalloenzymes (e.g., laccase) that are strongly upregulated in symbiotic hyphae. (C) 2010 Elsevier Inc. All rights reserved.
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