期刊
FUNGAL GENETICS AND BIOLOGY
卷 46, 期 1, 页码 55-66出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2008.10.006
关键词
Peroxisomal ABC transporters; beta-Oxidation; Peroxisomes; Mitochondria; Filamentous fungi
资金
- Centre National de la Recherche Scientifique
- University of Paris-Sud [UMR 8621]
ATP-binding cassette transporters are ubiquitous proteins that facilitate transport of diverse substances across a membrane. However, their exact role remains poorly understood. in order to test their function in a fungus life cycle, we deleted the two Podospora anserina peroxisomal ABC transporter pABC1 and pABC2 genes as well as the three genes involved in peroxisomal (fox2) and mitochondrial (scdA and echA) P-oxidation. Analysis of the single and double mutants shows that fatty acid p-oxidation occurs in both organelles. Furthermore, the peroxisomal and mitochondrial fatty acid beta-oxidation pathways are both dispensable for vegetative and sexual development. They are, however, differently required for ascospore pigmentation and germination, this latter defect being restored in a Delta pABC1 and Delta pABC2 background. We report also that lack of peroxisomal ABC transporters does not prevent peroxisomal long-chain fatty acid oxidation, suggesting the existence of another pathway for their import into peroxisomes. Finally, we show that some aspects of fatty acid degradation are clearly fungus species specific. (C) 2008 Elsevier Inc. All rights reserved.
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