期刊
FUNGAL GENETICS AND BIOLOGY
卷 46, 期 9, 页码 682-687出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2009.05.001
关键词
Cryptococcus neoformans; Sec4/Rab8; Secretion; Bafilomycin A; Ultrastructure; GXM; Organelle; Electron microscopy; Yeast; Fungi
资金
- NIH [A1073380]
- Department of Molecular Microbiology
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI073380] Funding Source: NIH RePORTER
Cryptococcus neoformans is a basidiomycete that causes deadly infections in the immunocompromised. We previously generated a secretion mutant in this fungus by introducing a mutation in the SAVI gene, which encodes a homolog of the Sec4/Rab8 subfamily GTPases. Under restrictive conditions there are two notable morphological changes in the sav1 mutant: accumulation of post-Golgi vesicles and the appearance of an unusual organelle, which we term the sav1 body (SB). The SB is an electron-transparent structure 0.2-1 mu m in diameter, with vesicles or other membranous structures associated with the perimeter. Surprisingly, the SB was heavily labeled with anti-glucuronoxylomannan (GXM) antibodies, suggesting that it contains a secreted capsule component, GXM. A structure similar to the SB, also labeled by anti-GXM antibodies, was induced in wild type cells treated with the vacuolar-ATPase inhibitor, bafilomycin A,. Bafilomycin A, and other agents that increase intraluminal pH also inhibited capsule polysaccharide shedding and capsule growth. These studies highlight an unusual organelle observed in C neoformans with a potential role in polysaccharide synthesis, and a link between luminal pH and GXM biosynthesis. (c) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据