4.4 Article

Carbon source affects PKA-dependent polarity of Neurospora crassa in a CRE-1-dependent and independent manner

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FUNGAL GENETICS AND BIOLOGY
卷 45, 期 2, 页码 103-116

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ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2007.05.005

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polarity; carbon catabolite repression; conidial germination; hyphae; fructose; aberrant splicing

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A defect in mcb, encoding the cAMP-dependent protein kinase A (PKA) regulatory subunit in Neurospora crassa, which confers an apolar growth phenotype, is accompanied by an increase in PKA activity levels. Both PKA and CRE-1 [a key carbon catabolite repression (CCR) regulator] mediate the cellular response to carbon-source availability. Inactivation of the cre-1 gene resulted in reduced growth rate; abnormal hyphal morphology and altered CCR. Both PKA and CRE-1 affected morphology in a carbon-dependent manner, as fructose suppressed the apolar morphology of the mcb strain and enabled faster growth of the. Delta cre-1 mutant. An increase in cre-1 transcript abundance was observed in mcb and a reduction in PKA activity levels was measured in Delta cre-1. CRE-1 is involved in determining PKA-dependent polarity, as an mcb; Delta cre-1 strain displayed partial reestablishment of hyphal polarity. Taken together, our results demonstrate regulatory interactions between PKA and CRE-1 that affect cell polarity in a filamentous fungus. (c) 2007 Elsevier Inc. All rights reserved.

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