期刊
FUNGAL GENETICS AND BIOLOGY
卷 45, 期 2, 页码 94-102出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2007.10.001
关键词
aspergillus; nitrite; nitrate; transport; nrtA; nrtB; NitA
Disruption of the Aspergillus nidulans high-affinity nitrate transporter genes (nrtA and nrtB) prevents growth on nitrate but not nitrite. We identified a distinct nitrite transporter (K-m = 4.2 +/- 11 mu M, V-max = 168 +/- 21 nmol mg(-1) DW-1 h(-1)), designated NitA. Disruption of nrtA, nrtB and nitA blocked growth on nitrite, despite low rates of nitrite depletion we ascribe to passive nitrous acid permeation. Growth of the single mutant nitA16 on nitrite was wild-type, suggesting that NrtA and/or NrtB transports nitrite as well as nitrate. Indeed. NrtA and NrtB transport nitrite at higher rates than NitA; K-m and V-max values were 16 +/- 4 mu M and 808 +/- 67 nmol mg(-1) DW-1 h(-1) (NrtA) and 11 +/- 1 mu M and 979 +/- 17 nmol mg(-1) DW-1 h(-1) (NrtB). We suggest that NrtA is a nitrate/nitrite transporter, NrtB absorbs nitrite in preference to nitrate and NitA is exclusively a nitrite transporter. Crown copyright (c) 2007 Published by Elsevier Inc. All rights reserved.
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