期刊
FUNGAL GENETICS AND BIOLOGY
卷 45, 期 7, 页码 1062-1074出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2008.04.003
关键词
signal transduction; HOG-like; MAPK; histidine kinase; Botrytis; fungicide resistance; osmotic stress; oxidative stress; pathogenicity; conidiation
In filamentous ascomycetes, HOG-like signal transduction cascades are involved in the resistance to hyper-osmotic conditions and to dicarboximides and phenylpyrroles. The histidine kinase (HK) Bos1 and the mitogen-activated protein kinase (MAPK) Sak1 are important for the adaptation to hyper-osmotic and oxidative stress, development, and pathogenicity in the phytopathogenic fungus Botrytis cinerea. However, bos1 Delta and sak1 Delta mutants created previously, also presented different phenotypes, especially the sak1 Delta mutants were not resistant to high fungicide concentrations. Since both single mutants were constructed in different parental strains, phenotypic variations due to the genetic background might be suspected. In order to establish the relationship between both protein kinases, we analyzed Sak1 phosphorylation under the control of the Bos1 HK and we realized epistasis analysis between bos1 Delta and sak1 Delta mutations through the construction of isogenic single and double mutants. Our results show that Bos1 negatively regulates Sak1 phosphorylation and that Bos1 regulates certain phenotypes independently of Sak1. They include fungicide susceptibility, adaptation and conidiation on high neutral osmolarity. (C) 2008 Elsevier Inc. All rights reserved.
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